james.lucas (at) ucsf.edu
I am interested in utilizing molecular biology techniques to investigate protein structure-function relationships as well as developing Rosetta to engineer tools for synthetic biology. I am currently developing a general protocol for designing analyte-induced dimerization domains coupled with split-protein reporters as reliable in vivo biosensors.
NSF Graduate Fellow
- Kundert K, Lucas JE, Watters KE, Fellmann C, Ng AH, Heineike BM, Fitzsimmons CM, Oakes BL, Qu J, Prasad N, Rosenberg OS, Savage DF, El-Samad H, Doudna JA, Kortemme T. Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs. Nat Commun. 2019 May 9;10(1):2127. doi: 10.1038/s41467-019-09985-2.
- Barlow KA, Ó Conchúir S, Thompson S, Suresh P, Lucas JE, Heinonen M, Kortemme T. Flex ddG: Rosetta Ensemble-Based Estimation of Changes in Protein-Protein Binding Affinity upon Mutation. J Phys Chem B. 2018 doi: 10.1021/acs.jpcb.7b11367.
- Lucas, JE, Siegel, JB. Quantitative functional characterization of conserved molecular interactions in the active site of mannitol 2-dehydrogenase. Protein Science, 24: 936–945, 2015. doi:10.1002/pro.2669